A nuclei were imaged using confocal microscopy following 24 h of incubation with i free sirna, ii gemini sirna, iii gpuretubes sirna, iv gisonanotubesm sirna and v gisonanotubess sirna complexes. Dotap functionalizing singlewalled carbon nanotubes as nonviral. Here, we summarize our previously developed protocols for functionalization and bioconjugation of single. Application and further development of nanomedicine strategies for advanced theranostics madaswamy s. In addition to the ability of cnts to act as carriers for a wide range of therapeutic molecules, their large surface. So certainly carbon nanotubes has a promising future. Modified carbon nanotubes cnts have been shown to protect sirna and facilitate its entry into cells. Ebbesen cylindrical carbon tubes and fibers were known,5 these nanotubes appeared perfectly graphitized and capped at each end with pentagons, just like the fullerene molecules. May 17, 2006 please use one of the following formats to cite this article in your essay, paper or report.
Design of cationic multiwalled carbon nanotubes as. In this study, the treatment of a human lung carcinoma model in vivo using sirna sequences leading to cytotoxicity and cell death is carried out using either cationic liposomes dotap. Properties of semiconducting and metallic carbon nanotubes. Pdf small interfering rnas sirna are promising tools for applications in gene silencing for therapy and functional analysis of genes. Types, methods of preparation and applications parijat pandey, mandeep dahiya department of pharmaceutical sciences, maharshi dayanan d university, rohtak, har yana, india. Carbon nanotubebased drug delivery has shown promise in various in vitro and in vivo experiments including delivery of small interfering rna sirna, paclitaxel and doxorubicin. The carbon network of the shells is closely related to the honeycomb arrangement of the carbon atoms.
Carbon nanotubes cnts are hollow fibrous carbon molecules that were first observed in the soot of arcburned graphite rods 11 figure 1. We recently demonstrated in a proofofprinciple study that aminofunctionalized multiwalled carbon nanotubes fmwnt can effectively deliver in vivo an sirna sequence, trigger. Carbon nanotube, singlewalled 98% semiconducting single. Nanotubes effectively deliver sirna to intact plant cells and protect. Moreover, singlewalled carbon nanotubes with various interesting intrinsic. The nanotubes may consist of one up to tens and hundreds of concentric shells of carbons with adjacent shells separation of 0. Owing to their high aspect ratio, large surface area, rich surface chemical functionality, and size stability at the nano scale, cnts have been extensively. Biomedical applications of carbon nanotubes have attracted much attention in recent years. Sep 20, 2011 sirna delivery with functionalized carbon nanotubes. Singlewalled carbon nanotubes cnt has been used for nucleic acid delivery such as sirna delivery.
Here, noncovalent functionalization of cnts is performed and shown to. A new series of dendronfunctionalized multiwalled carbon nanotube mwnt derivatives, characterized by the presence of numerous positively charged tetraalkyl ammonium salts at the periphery of the dendron, has been synthesized. However, simple and efficient methods to functionalize cnts are needed. Development of noncovalent functionalization of carbon. Functionalized singlewalled carbon nanotubes swnt with 1,2 dioleoyl3. Preparation of carbon nanotube bioconjugates for biomedical. Sirna delivery with functionalized carbon nanotubes request pdf. Graphite is made up of layers of carbon atoms arranged in a hexagonal lattice, like chicken wire see.
Noncovalent functionalization of carbon nanotubes for sirna. Request pdf sirna delivery with functionalized carbon nanotubes carbon nanotubes cnts have been studied for drug, antigen and nucleic acid delivery. Introduction to carbon nanotubes carbon nanotubes were discovered in 1991 by sumio iijima of nec and are effectively long, thin cylinders of graphite, which you will be familiar with as the material in a pencil or as the basis of some lubricants. Since the seminal report on use of graphene oxide go as an efficient nanocarrier for drug delivery by dai et al. Mar 27, 2014 rna interference rnai therapy is promising for treating various diseases but the delivery of small interfering rna sirna is difficult. Carbon can bond in different ways to construct structures with completely different properties. Sigmaaldrich offers a number of carbon nanotube, singlewalled products. Carbon nanotubes cnts have been introduced recently as a novel carrier system for both small and large therapeutic molecules. Specifically, singlewalled carbon nanotubes swcnt have been shown to deliver sirna to tumors in vivo. To overcome the technical difficulties of sirna delivery. Applications of functionalized carbon nanotubes for the.
However, delivery to specific cells and tissues in vivo presents significant challenges. Nano and microtechnology emerging impact on pharmacology and. Although the cultured cell model allows us to elucidate the detailed uptake of carbon nanotube dna sirna complexes by cells, it does not tell us how good the tissue uptake is when delivered through the systemic circulation, which is the crucial step in the practical use of the carbon nanotube dna sirna complexes. Carbon nanotubes are unique tubular structures of nanometer diameter and large lengthdiameter ratio. Degradable lipid nanoparticles with predictable in vivo. Carbon nanotubes cnts have been studied for drug, antigen and nucleic acid delivery both in vitro and in vivo. Rna polymer polyru were formed through a nonspecific binding mechanism which could allow for the dissociation of the polyru from the swnts upon delivery. Functional delivery of sirna in mice using dendriworms. Carbon nano tubes have been proposed as novel nanomaterials that can offer signi. Sep 20, 2011 carbon nanotubes cnts have been studied for drug, antigen and nucleic acid delivery both in vitro and in vivo. Cnt based sirna delivery is directly proportional to the enhanced sirna. Please use one of the following formats to cite this article in your essay, paper or report. Tankeshwar ab and kihyun kim c a department of bio and nano technology, guru jambheshwar university of science and technology, hisar, haryana 125001, india. Carbon nanotubes are classified as singlewalled carbon nanotubes swnts and multiwalled carbon nanotubes mwnts, depending on the number of.
Sirna delivery with functionalized carbon nanotubes. Sandeep kumar a, ruma rani a, neeraj dilbaghi a, k. Due to their nano needle structure, they are supposed to cross the plasma membrane and enter directly into the cytoplasm likely upon an endocytosisindependent mechanism without inducing cell death. Dendrimer modified swcnts for high efficient delivery and. Rna interference rnai therapy is promising for treating various diseases but the delivery of small interfering rna sirna is difficult. Carbon nanotubes are novel nanomaterials that are thought to offer potential benefits to a variety of biomedical and clinical applications.
Multi walled carbon nano tubes induced hepatotoxicity in swiss albino mice. Carbon nanotubes, purification techniques for carbon nanotubes. Sep 20, 2011 read sirna delivery with functionalized carbon nanotubes, international journal of pharmaceutics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Characteristics and properties of single and multi walled carbon nanotubes. Varkouhi ak1, foillard s, lammers t, schiffelers rm, doris e, hennink we, storm g. Herein, we develop a nanotubebased platform for direct delivery of sirna, and show high silencing efficiency in intact plant cells.
Sirna delivery with functionalized carbon nanotubes sciencedirect. Antitumor activity and prolonged survival by carbon. Camino center for functional nanomaterials, brookhaven national laboratory, upton, ny 11973 proposal title. Most important of all, iijima noticed that the carbon atoms in each nano tubes closed shells were ar. The low toxicity, the excellent membrane penetration ability, the protection. Carbon nanotubes, whose walls are just 1 atom thick, with diameters of only 1 to 2 nm, seems to be one of the perfect candidates to take us right to the end of moores law curve. Over the past many decades there has been a sudden growth in the field of research and advancement related to the nanomaterials. The translocation of singlewalled carbon nanotubes swnts across mcf7 breast cancer cells was demonstrated with radioisotope labeling. Uptake of gsingle wall carbon nanotubes sirna complexes into purified nuclei. The conjugation of sirna to functionalized carbon nanotubes fcnts significantly increases their delivery into cells due to cnt uptake through mechanisms other than endocytosis 10. Nano chemistry is an advance area of chemistry for the study of nanoparticles and their compounds reactions and the production.
Swnts can be conjugated with small interfering rna sirna or loaded with. Review nanotheranostics application and further development. In conclusion, dendrimer pamam modified single walled carbon nanotubes can be used for high efficient delivery and intracellular imaging of sirna, and own great potential in applications such as gene or drug delivery and tumor targeted imaging and simultaneous therapy in near future. Carbon nanotubes deliver sirna into human cells nanowerk news short pieces of rna, known as small interfering rna sirna, have the potential to become a new class of anticancer drugs if researchers can solve the problem of how to deliver these fragile molecules to cancer cells. Rna polymer translocation with singlewalled carbon nanotubes. Delivery of telomerase reverse transcriptase small interfering. Nano biotechnology list of high impact articles ppts. Characteristics and properties of single and multi walled. Reciprocal effects of the chirality and the surface functionalization on the drug delivery permissibility of carbon nanotubes. Some are topically administered for transdermal drug delivery and as eye drops, such as restasis containing cyclosporine a for chronic dry eye. Selective nuclear localization of sirna by metallic versus. Fabrication of carbon nanotube fieldeffect transistors with semiconductors as source and drain contact materials z. Cnt were characterized and sirna delivery capacities of the cnts. One of the most significant challenges in the development of clinically viable delivery systems for rna interference therapeutics is to understand how.
Carbon nanotubes in cancer therapy and drug delivery. Nanotubes effectively deliver sirna to intact plant cells. Synthesis and characterization of a carbon nanotube. Carbon nanotubes cnts are attractive nanostructures that. Among all the nanomaterials, a material by name carbon nanotubes has led the way in terms of its captivating structure and also its ability to furnish specific applications ranging from the electronics to the energy. Wei shao, paul arghya, mai yiyong, laetitia rodes and satya prakash may 9th 20. Functionalized singlewalled carbon nanotubes swnt with 1,2dioleoyl3. The positive charges on the mwnt surface, coupled with the unique ability of carbon nanotubes cnts to penetrate cell membranes, make the new derivatives potentially. Carbon nanotubes have many unique physical and chemical properties that are being widely explored for potential applications in biomedicine especially as transporters of drugs, proteins, dna and rna into cells. Enhanced cellular internalization and gene silencing with a. Carbon nanotubes exist as a macromolecule of carbon, analogous to a sheet of graphite, rolled into a cylinder. Graphite in its molecular form looks like a sheet of chicken wire, a tessellation of hexagonal rings of carbon. Look at the background when coiled, the carbon arrangement becomes exceptionally strong. Purification techniques for carbon nanotubes including gas and liquid phase and intercalation.